UC Davis Comprehensive Course in Flow Cytometry - Cancer Center Scholarship Applicants 2020

Apply Now! Flow Cytometry Training Course scholarships for UC Davis Comprehensive Cancer Center members are now available!  

 

Details: Cancer Center members are eligible to apply for five (5) full tuition scholarships ($875.00) for the UC Davis Comprehensive Course in Flow Cytometry, offered ONLINE August 24-28, 2020 by the Comprehensive Cancer Center’s Flow Cytometry Shared Resource.

Who can apply? Scholarships are available to faculty, post-doctoral researchers, graduate student researchers, undergraduate students and laboratory staff members who are Cancer Center members or who work for Cancer Center member investigators.

How to apply? Applicants need to submit a short, 1 paragraph description of their research project and indicate specifically:

·      How this flow cytometry training will advance their research goals.

·      Describe plans to use the Flow Cytometry Core’s instrumentation to carry out their research work (Sacramento or Davis).

·      Agreement to appropriately cite the UC Davis Flow Cytometry Shared Resource and the Cancer Center Shared Grant when publishing cytometry data generated using core instrumentation and supported by this Cancer Center educational opportunity.  

Key dates: Applications are currently being accepted until August 9, 2020 (Sunday evening). We will notify you in mid-August whether you have been selected to receive a scholarship. You may wish to register for the course now at full price to ensure a seat – the full lecture + laboratory option sells out each year.  If you are accepted to receive a scholarship, your tuition fee will be refunded to you.

You may wish to register at full price to ensure a seat.  If you are subsequently accepted, the full registration fee will be refunded to you. To register, go to https://registration.genomecenter.ucdavis.edu/events/flow_cytometry_2020/

The course objectives are:

·      Learn the fundamental concepts of how flow cytometers work

·      Explain the physical and electronic concepts in light collection and signal processing

·      Discuss best practices in sample preparation for flow cytometry

·      Describe standard assays and techniques

·      Introduce new cutting edge technologies and applications for single cell analysis and genomics

·      Demystify multicolor cell staining, compensation and analysis

Laboratory sessions include:

·      UC Davis LSRII: Software setup for successful multicolor acquisition and analysis

·      UC Davis Astrios Cell Sorter: Cell sorting fundamentals using the Beckman Coulter Astrios

·      Cytometer setup and quality control for consistent performance

·      Principles and Advantages of Spectral Cytometry on the Cytek Aurora

·      Exosome and small particle detection using CCD detectors on the Amnis CellStream Cytometer

·      Multicolor Panel Design workshop with BD Biosciences, don’t miss this!

·      FlowJo basic and advanced flow cytometry data analysis and panel design wizard

·      And more!

The daily schedule from the 2018 course can be downloaded by clicking here

Who should attend?

Prior course participants have included faculty, post-doctoral researchers, graduate students, staff, motivated high school students (!) and researchers from our industry partners.

What are the prerequisites?

Flow cytometry is now used across a broad spectrum of biological research programs. As such, there are no prerequisites other than familiarity with general biological concepts and a desire to learn about fluorescence, flow cytometry and cell sorting. Some general laboratory experience in working with live cells is helpful, particularly immunofluorescence microscopy and cell staining.

What do I need to bring? The lecture materials will be taught online using teleconferencing software (Zoom,  Slack) and a copy of the lecture materials will be provided in a PDF format. It is necessary to have a laptop or tablet (but not required) to view the lectures, take notes and follow along with the lecture materials. We will be working with FlowJo software in the data analysis demonstrations (during lecture sessions) and in the FlowJo lab. FlowJo version 10 will work on either Mac or PC and you can download a free trial during the course to use if you wish.

Can I provide my own samples? The laboratory sessions will use previously prepared samples geared toward specific teaching goals, so we won’t have time to work with individual samples.

Can I bring my own data? We will provide experimental data sets during the workshop to keep everyone on track and to illustrate important concepts. If you have specific questions, please bring them, or flow plots from your own analysis or a print out for us to look over and offer advice. During the latter part of the week we’ll cover multicolor panel design, you are encouraged to share your proposed multicolor stain panel details with us as we work to build panels in an interactive exercise. 

Where is the course? The course is held online via Zoom and Slack.

 

Questions?

If you have any questions, please contact Bridget at bmclaughlin@ucdavis.edu