So you want to do a: Single Cell RNA-seq experiment
Nov. 6, 2017, 1 p.m. - Nov. 6, 2017, 3 p.m.
Organizer -
Bioinformatics Core
Contact -
UC Davis Bioinformatics Core, training.bioinformatics@ucdavis.edu
Location -
UC Davis, Genome and Biomedical Sciences Facility Auditorium (GBSF Rm. 1005)
This FREE workshop is intended for those who are interested in and are in the planning stages of conducting a single-cell RNA-Seq experiment. Topics to be discussed will include:
- Experimental Design of scRNA-Seq experiments
- Sample preparation, best practices
- Single cell library prep and High throughput sequencing basics and choices
- Cost estimation
- Single Cell Analysis
- Data cleanup and quality assurance
- Mapping data
- Assigning reads to cells and genes and counting
- Analysis of single cell transcriptomics, tSNE plots, clustering, comparisons across samples
- Downstream analysis/visualizations and tables