Real-time PCR Readiness Workshop and Wet Lab
Sept. 19, 2016, 9 a.m. - Sept. 23, 2016, 2 p.m.
Real-time PCR Core Facility
Samantha Barnum, Cara Wademan
GBSF 1005 (lecture), 2411 Tupper Hall (wet lab)
Real-time polymerase chain reaction is becoming more and more important in almost all laboratories. This course covers an in-depth view of the theoretical and practical knowledge of quantitative PCR techniques. Topics include molecular diagnostics in single-cells, high throughput analysis in qPCR, minimum information for publication of qPCR experiments (MIQE) guidelines, small RNAs, pre-analytical steps, and qPCR biostatistics and bioinformatics. The course will also examine the latest developments, opportunities and applications of both digital PCR and qPCR through case studies across diverse areas. Participants will learn about qPCR theoretical concepts, experimental design, primers/probe design, applications, sample preparation and extraction, quality controls of gDNA and total RNA, contamination control and monitoring for amplification reactions, optimization and troubleshooting of qPCR, gene expression, mRNA, quantification strategies in RT-qPCR, digital PCR, NGS, and data analysis and interpretation.
qPCR has substantial advantages in quantifying low target copy numbers from limited amounts of tissue or identifying minor changes in mRNA or microRNA expression levels in samples with low RNA concentrations or from single cells analysis. It is essential to have a comprehensive understanding of the underlying basic principles, error sources and general problems inherent with qPCR and RT-qPCR.
This course is aimed at giving participants a deep and objective understanding of real-time quantitative PCR, expression profiling, its applications, and biostatistics. The course is intended for academic or industrial persons considering working with qPCR or scientists currently working with qPCR seeking a deeper understanding.
The wet lab portion of the course is designed to provide hands-on training. Participants will be able to apply the knowledge they gained during the course talks in a practical laboratory setting. PLEASE NOTE A CHANGE IN TIME FOR THE WET LAB! The wet lab will now be from 2 – 5pm, September 21st and 8:30am-noon September 22nd and 23rd.
Student - Lecture and Wet Lab: $360
Academic - Lecture and Wet Lab: $480
Industry - Lecture and Wet Lab: $600