UC Davis Comprehensive Course in Flow Cytometry - Full Workshop

This comprehensive flow cytometry course covers flow cytometry basics and advanced concepts in lecture sessions led by UC Davis Flow Cytometry Core laboratory staff. Cytometry theory is put into practice in interactive laboratory sessions using many of our UC Davis cytometers plus instrumentation and software provided by our vendor partners to teach you to a variety of established and new approaches. Special guest speakers will introduce new trends in flow cytometry. Thanks to many generous sponsors, lunches and coffee breaks are provided to promote opportunities for interaction with the instructors and cytometry specialists. Please make plans to join us this summer for another fun week of flow education.

A limited number of scholarships are available for persons affiliated with UC Davis Comprehensive Cancer Center member labs.  See https://registration.genomecenter.ucdavis.edu/events/flow_cytometry_2019_CancerCenter_ScholarshipApplicant/ for more information.

There are two options for attendance:

Full curriculum: Lecture plus afternoon laboratory sessions – limited to 32 participants 

Lecture only (All day Monday, mornings only Tu-Friday) – 20 participants (If you would like to register for lecture only, please go to https://registration.genomecenter.ucdavis.edu/events/flow_cytometry_2019_lecture_only/)

 

The course objectives are:

·      Learn the fundamental concepts of how flow cytometers work

·      Explain the physical and electronic concepts in light collection and signal processing

·      Discuss best practices in sample preparation for flow cytometry

·      Describe standard assays and techniques

·      Introduce new cutting edge technologies and applications for single cell analysis and genomics

·      Demystify multicolor cell staining, compensation and analysis

Laboratory sessions include:

·      UC Davis LSRII: Software setup for successful multicolor acquisition and analysis

·      UC Davis Astrios Cell Sorter: Cell sorting fundamentals using the Beckman Coulter Astrios

·      Cytometer setup and quality control for consistent performance

·      Principles and Advantages of Spectral Cytometry on the Cytek Aurora

·      Exosome and small particle detection using CCD detectors on the Amnis CellStream Cytometer

·      Multicolor Panel Design workshop with BD Biosciences, don’t miss this!

·      FlowJo basic and advanced flow cytometry data analysis and panel design wizard

·      And more!

 

Special guest speakers:

·      Holden Maecker, Ph.D., Director, Stanford University Human Immune Monitoring Core

·      Jolene Bradford, Associate Director Flow Cytometry Systems, Molecular Probes, a Division of Invitrogen with ThermoFisher Scientific

·      Marcia Mednick, Senior Applications Support Scientist, Takara Bio

 

The daily schedule from the 2018 course can be downloaded by clicking here

 

Who should attend?

Prior course participants have included faculty, post-doctoral researchers, graduate students, staff, motivated high school students (!) and researchers from our industry partners.

 

What are the prerequisites?

Flow cytometry is now used across a broad spectrum of biological research programs. As such, there are no prerequisites other than familiarity with general biological concepts and a desire to learn about fluorescence, flow cytometry and cell sorting. Some general laboratory experience in working with live cells is helpful, particularly immunofluorescence microscopy and cell staining.

 

What do I need to bring? The lecture materials will be provided in a PDF format, it is helpful to have a laptop or tablet (but not required) to take notes and follow along with the lecture materials. We will be working with FlowJo software in the data analysis demonstrations (during lecture sessions) and in the FlowJo lab. FlowJo version 10 will work on either Mac or PC and you can download a free trial during the course to use if you wish.

 

Can I provide my own samples? The laboratory sessions will use previously prepared samples geared toward specific teaching goals, so we won’t have time to work with individual samples.

 

Can I bring my own data? We will provide experimental data sets during the workshop to keep everyone on track and to illustrate important concepts. If you have specific questions, please bring them, or flow plots from your own analysis or a print out for us to look over and offer advice. During the latter part of the week we’ll cover multicolor panel design

 

How much does it cost?

Registration fees include light breakfast, lunch and snacks, but do not include dinner, lodging, travel or parking fees. We do offer a lecture only option but encourage participation in the full lecture + laboratory option for the best experience.

 

For the full lecture plus laboratory curriculum, the registration fees are as follows:

·      UC Davis faculty, student or staff: $975.00

·      Non UC Davis Academic or Not-For-Profit participants: $1,200.00

·      Industry participants: $1,450.00

 

For the lecture only option, the registration fees are:

·      UC Davis faculty, student or staff: $500.00

·      Non UC Davis Academic or Not-For-Profit participants: $650.00

·      Industry participants: $1,000.00

 

Where is the course? The course is held in Davis, CA. The specific location will be provided at a later date.

 

Questions?

If you have any questions, please contact Bridget at bmclaughlin@ucdavis.edu