2017 Proteomics Short Course

This hands-on introductory proteome analysis workshop is designed to expose participants to fundamental technology platforms and current methodologies in the field of mass spectrometry-based proteomics.

Topics covered include:

• Bottom-up quantitative and non-quantitative nano-electrospray proteomics tandem mass spectrometry (nESI-LC/MSMS)
• In-gel digestion lab class
• Nano-scale HPLC & peptide spectrum matching using open-source software (X! Tandem)
• Targeted proteomics
• de novo sequencing
• Data analysis, using programs such as Scaffold and Skyline

Lectures will be presented by the proteomics core staff as well as leading proteomics experts including scientists from UC Davis, UCSF, Stanford, Bruker, Thermo Scientific and the Buck institute. This is an intensive, practical course that is meant to give the participant enough background to allow them to take the course material and build upon and apply it to their own individual projects. No proteomics experience is necessary, although laboratory experience working with proteins is usually helpful.

Specific course topics may change due to sample and mass spectrometer availability.

Enrollment is limited to 30 students (lecture/lab), 40 lecture only.