Phase Genomics Seminar
Sept. 19, 2017, 2:30 p.m. - Sept. 19, 2017, 3:30 p.m.
UC Davis, GBSF Auditorium
Phase Genomics creates genome and metagenome assemblies by using Hi-C to measure the physical structure of DNA molecules in vivo, bringing more than just sequence information to bear in genome assembly. Phase Genomics offers an easy way to apply the power of Hi-C to your genomic and metagenomic projects. Come to this seminar to learn more!
How Hi-C is transforming genome and metagenome assembly
Chromosome conformation capture methods like Hi-C measure the 3D organization of DNA in vivo using a combination of crosslinking, proximity-ligation, and paired-end sequencing.
Because this method captures genomic contiguity on intact chromosomes, the resultant information can be used to generate end-to-end chromosome-scale scaffolds for large genomes. Since Hi-C junctions form within intact cells, any sequences interacting by Hi-C must have originated from the same species/strain in a mixed population, enabling metagenomic deconvolution.
Capturing genomic proximity information in vivo removes several major obstacles in genome and metagenome assembly, improving the quality and efficiency of genome discovery efforts.